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pcmv ha n vector  (TaKaRa)


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    Structured Review

    TaKaRa pcmv ha n vector
    Pcmv Ha N Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 764 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcmv+ha+n+vector/pCMV-HA+Vector+Set/10__3389_slash_av__2026__15087-99-9-11
    Average 96 stars, based on 764 article reviews
    pcmv ha n vector - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Mutagenesis:

    Article Title: Evidence for existence of an apoptosis-inducing BH3-only protein, sayonara, in Drosophila
    Article Snippet: The ccd AA mutant was newly synthesized by GenScript. .. The amino acid sequences of BH3 motif AA mutant and ccd AA mutant are the following: BH3 motif AA mutant, EAYNEGVEAAAEK (122–134); ccd AA mutant, ccd1, GGGTGGGGGTGGGSYGGGNGG (162–182), ccd2, GTGGGGSGGGQGGTGGGTGGG (199–219) For HA-tagged Synr, synr WT, DBH3, and BH3AA mutants were once inserted into the pCMV-HA-N vector (Takara #635690). ..

    Clone Assay:

    Article Title: FLT3 Inhibitors Induce p53 Instability, Driven by STAT5/MDM2/p53 Competitive Interactions in Acute Myeloid Leukemia.
    Article Snippet: Full-length human wild-type MDM2 cDNA was cloned into the pFlagCMV2 vector (Sigma-Aldrich, St Louis, MO). .. Full-length human wild-type STAT5A and STAT5B was cloned into the pCMV-Myc-N vector (Clontech, Mountainview, CA, USA), whereas full-length human wild-type ubiquitin were cloned into the pCMV-HA-N vector (Clontech, Mountain View, CA). .. STAT5 siRNA was purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

    Article Title: Novel insights into the ULK2-FIP200-AMPK-mediated regulation of autophagy and BCR::ABL degradation in chronic myeloid leukemia.
    Article Snippet: Chronic myeloid leukemia (CML) is a myeloproliferative neoplasm driven by the BCR::ABL fusion tyrosine kinase.. AMP-activated protein kinase (AMPK) plays a pivotal role in regulating cellular energy homeostasis, ensuring an adequate ATP supply for CML cell proliferation.. ULK1, a well-known AMPK substrate, is a critical serine/threonine kinase in the autophagy initiation complex.

    Ubiquitin Proteomics:

    Article Title: FLT3 Inhibitors Induce p53 Instability, Driven by STAT5/MDM2/p53 Competitive Interactions in Acute Myeloid Leukemia.
    Article Snippet: Full-length human wild-type MDM2 cDNA was cloned into the pFlagCMV2 vector (Sigma-Aldrich, St Louis, MO). .. Full-length human wild-type STAT5A and STAT5B was cloned into the pCMV-Myc-N vector (Clontech, Mountainview, CA, USA), whereas full-length human wild-type ubiquitin were cloned into the pCMV-HA-N vector (Clontech, Mountain View, CA). .. STAT5 siRNA was purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

    Construct:

    Article Title: Identification of ATG7 as a protein ATG8ylation substrate.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pmRFP1-EGFP-LC3 MiaoLingBio P48062 pX333-EGFP vector HedgehogBio HH-CAS-153 pET28a(+)-3xFlag-LC3B-pro-6xHis mutants This study N/A pET28a(+)-Flag-ATG4B-6xHis This study N/A pET28a(+)-ATG7-TST This study N/A pCDFDuet-12xHis-SUMO-3xFlag-LC3B Q116P G120 This study N/A pCDFDuet-12xHis-SUMO-3xFlag-LC3B Q116P G120∼GST-HA-ATG3 This study N/A Software and algorithms Word, and PowerPoint Microsoft https://www.office.com ImageJ Wayne Rasband (NIH) https://imagj.net Prism8 GraphPad https://www.graphpad.com Cell Reports 44, 116485, November 25, 2025 19 GFP-tagged ATG10, ATG5 and ATG12 were generated by cloning their CDS into pLVX-AcGFP1-C1 vector (Clontech). .. Constructs of HA-tagged ATG3 and ATG7 generated by cloning them into pCMV-HA-N vector (Clontech). ..

    Generated:

    Article Title: Identification of ATG7 as a protein ATG8ylation substrate.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pmRFP1-EGFP-LC3 MiaoLingBio P48062 pX333-EGFP vector HedgehogBio HH-CAS-153 pET28a(+)-3xFlag-LC3B-pro-6xHis mutants This study N/A pET28a(+)-Flag-ATG4B-6xHis This study N/A pET28a(+)-ATG7-TST This study N/A pCDFDuet-12xHis-SUMO-3xFlag-LC3B Q116P G120 This study N/A pCDFDuet-12xHis-SUMO-3xFlag-LC3B Q116P G120∼GST-HA-ATG3 This study N/A Software and algorithms Word, and PowerPoint Microsoft https://www.office.com ImageJ Wayne Rasband (NIH) https://imagj.net Prism8 GraphPad https://www.graphpad.com Cell Reports 44, 116485, November 25, 2025 19 GFP-tagged ATG10, ATG5 and ATG12 were generated by cloning their CDS into pLVX-AcGFP1-C1 vector (Clontech). .. Constructs of HA-tagged ATG3 and ATG7 generated by cloning them into pCMV-HA-N vector (Clontech). ..

    Article Title: Evidence for existence of an apoptosis-inducing BH3-only protein, sayonara, in Drosophila
    Article Snippet: .. We also generated HA-Debcl with pCMV-HA-N vector (Takara #635690). .. Myc tag was also added at the N terminus of synr with pCMV-Myc vector (Takara #635689).

    Cloning:

    Article Title: Identification of ATG7 as a protein ATG8ylation substrate.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pmRFP1-EGFP-LC3 MiaoLingBio P48062 pX333-EGFP vector HedgehogBio HH-CAS-153 pET28a(+)-3xFlag-LC3B-pro-6xHis mutants This study N/A pET28a(+)-Flag-ATG4B-6xHis This study N/A pET28a(+)-ATG7-TST This study N/A pCDFDuet-12xHis-SUMO-3xFlag-LC3B Q116P G120 This study N/A pCDFDuet-12xHis-SUMO-3xFlag-LC3B Q116P G120∼GST-HA-ATG3 This study N/A Software and algorithms Word, and PowerPoint Microsoft https://www.office.com ImageJ Wayne Rasband (NIH) https://imagj.net Prism8 GraphPad https://www.graphpad.com Cell Reports 44, 116485, November 25, 2025 19 GFP-tagged ATG10, ATG5 and ATG12 were generated by cloning their CDS into pLVX-AcGFP1-C1 vector (Clontech). .. Constructs of HA-tagged ATG3 and ATG7 generated by cloning them into pCMV-HA-N vector (Clontech). ..

    Article Title: Novel insights into the ULK2-FIP200-AMPK-mediated regulation of autophagy and BCR::ABL degradation in chronic myeloid leukemia.
    Article Snippet: Chronic myeloid leukemia (CML) is a myeloproliferative neoplasm driven by the BCR::ABL fusion tyrosine kinase.. AMP-activated protein kinase (AMPK) plays a pivotal role in regulating cellular energy homeostasis, ensuring an adequate ATP supply for CML cell proliferation.. ULK1, a well-known AMPK substrate, is a critical serine/threonine kinase in the autophagy initiation complex.

    Plasmid Preparation:

    Article Title: Identification of ATG7 as a protein ATG8ylation substrate.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER pmRFP1-EGFP-LC3 MiaoLingBio P48062 pX333-EGFP vector HedgehogBio HH-CAS-153 pET28a(+)-3xFlag-LC3B-pro-6xHis mutants This study N/A pET28a(+)-Flag-ATG4B-6xHis This study N/A pET28a(+)-ATG7-TST This study N/A pCDFDuet-12xHis-SUMO-3xFlag-LC3B Q116P G120 This study N/A pCDFDuet-12xHis-SUMO-3xFlag-LC3B Q116P G120∼GST-HA-ATG3 This study N/A Software and algorithms Word, and PowerPoint Microsoft https://www.office.com ImageJ Wayne Rasband (NIH) https://imagj.net Prism8 GraphPad https://www.graphpad.com Cell Reports 44, 116485, November 25, 2025 19 GFP-tagged ATG10, ATG5 and ATG12 were generated by cloning their CDS into pLVX-AcGFP1-C1 vector (Clontech). .. Constructs of HA-tagged ATG3 and ATG7 generated by cloning them into pCMV-HA-N vector (Clontech). ..

    Article Title: Apple-derived extracellular vesicles modulate the expression of human intestinal bile acid transporter ASBT/SLC10A2 via downregulation of transcription factor RARα.
    Article Snippet: Purpose: Plant-derived extracellular vesicles (EVs) have been reported to exert biological activity on intestinal tissues by delivering their contents into intestinal cells.. We previously reported that ASBT/ SLC10A2 mRNA was downregulated by apple-derived extracellular vesicles (APEVs).. ASBT downregulation is effective in the treatment of cholestasis and chronic constipation, similar to the beneficial effects of apples.

    Article Title: Novel insights into the ULK2-FIP200-AMPK-mediated regulation of autophagy and BCR::ABL degradation in chronic myeloid leukemia.
    Article Snippet: Chronic myeloid leukemia (CML) is a myeloproliferative neoplasm driven by the BCR::ABL fusion tyrosine kinase.. AMP-activated protein kinase (AMPK) plays a pivotal role in regulating cellular energy homeostasis, ensuring an adequate ATP supply for CML cell proliferation.. ULK1, a well-known AMPK substrate, is a critical serine/threonine kinase in the autophagy initiation complex.

    Article Title: Identification of host proteins interacting with NS3 protein of Bluetongue virus
    Article Snippet: .. The DNA fragment NS3t was amplified and sub-cloned in pCMV-HA-N vector (Clontech, USA). ..

    Article Title: Evidence for existence of an apoptosis-inducing BH3-only protein, sayonara, in Drosophila
    Article Snippet: .. We also generated HA-Debcl with pCMV-HA-N vector (Takara #635690). .. Myc tag was also added at the N terminus of synr with pCMV-Myc vector (Takara #635689).

    Article Title: Notch signaling modulates Fgf23 expression through crosstalk with hypoxia and PTH pathways in osteogenic cells.
    Article Snippet: .. To generate the HA-tagged cAMP response element-binding protein (HA-CREB), CREB cDNA was amplified by PCR from a mouse embryonic day 15 cDNA library and subcloned into the pCMV-HA-N vector (Clontech). ..

    Polymerase Chain Reaction:

    Article Title: Apple-derived extracellular vesicles modulate the expression of human intestinal bile acid transporter ASBT/SLC10A2 via downregulation of transcription factor RARα.
    Article Snippet: Purpose: Plant-derived extracellular vesicles (EVs) have been reported to exert biological activity on intestinal tissues by delivering their contents into intestinal cells.. We previously reported that ASBT/ SLC10A2 mRNA was downregulated by apple-derived extracellular vesicles (APEVs).. ASBT downregulation is effective in the treatment of cholestasis and chronic constipation, similar to the beneficial effects of apples.

    Article Title: Notch signaling modulates Fgf23 expression through crosstalk with hypoxia and PTH pathways in osteogenic cells.
    Article Snippet: .. To generate the HA-tagged cAMP response element-binding protein (HA-CREB), CREB cDNA was amplified by PCR from a mouse embryonic day 15 cDNA library and subcloned into the pCMV-HA-N vector (Clontech). ..

    Amplification:

    Article Title: Identification of host proteins interacting with NS3 protein of Bluetongue virus
    Article Snippet: .. The DNA fragment NS3t was amplified and sub-cloned in pCMV-HA-N vector (Clontech, USA). ..

    Article Title: Notch signaling modulates Fgf23 expression through crosstalk with hypoxia and PTH pathways in osteogenic cells.
    Article Snippet: .. To generate the HA-tagged cAMP response element-binding protein (HA-CREB), CREB cDNA was amplified by PCR from a mouse embryonic day 15 cDNA library and subcloned into the pCMV-HA-N vector (Clontech). ..

    cDNA Library Assay:

    Article Title: Notch signaling modulates Fgf23 expression through crosstalk with hypoxia and PTH pathways in osteogenic cells.
    Article Snippet: .. To generate the HA-tagged cAMP response element-binding protein (HA-CREB), CREB cDNA was amplified by PCR from a mouse embryonic day 15 cDNA library and subcloned into the pCMV-HA-N vector (Clontech). ..



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